Journal:

Article Title: Apoptotic cell death in conjunction with CD80 costimulation confers uveal melanoma cells with the ability to induce immune responses

doi: 10.1046/j.1365-2567.2003.01632.x

Figure Lengend Snippet: Apoptotic cell death and costimulation induce proliferation of allogeneic lymphocytes. NR and apoptotic SOM 157d cells expressing CD80 or not were cocultured with PBMC from healthy donors in the presence (a) or absence (b) of DCs for 5 days and subsequent thymidine incorporation was determined (c.p.m.). Results show a representative of three independent experiments where bars represent the mean (± SD) of quadruplicates (NR = non-replicating). Percentage apoptotic cells after MMC treatment < 5% and after irradiation treatment > 40%.

Article Snippet: Separation of peripheral blood mononuclear cells from whole blood and DC generation Peripheral blood was obtained from healthy donors or from patients with uveal melanoma into heparin, and peripheral blood mononuclear cells (PBMC) isolated by standard gradient centrifugation in Lymphoprep (Nycomed, Pharmaceuticals Buckinghamshire, UK).

Techniques: Expressing, Irradiation

Journal:

Article Title: Apoptotic cell death in conjunction with CD80 costimulation confers uveal melanoma cells with the ability to induce immune responses

doi: 10.1046/j.1365-2567.2003.01632.x

Figure Lengend Snippet: Apoptotic tumour cells expressing CD80 promotes T cell activation and IFN-γ production. Melanoma cells were cocultured in the presence of DCs (a), or with PBMC alone (not shown) for a period of 5 days and lymphocyte activation was detected by flow cytometry [apoptotic (AP) and non-replicating (NR)]. ELISA-determined expression levels of IFN-γ (b). Lymphocyte activation results are represented as a mean of two donors ± SD. IFN-γ ELISA results represent the mean ± SD from two donors (each of which was assayed in duplicate).

Article Snippet: Separation of peripheral blood mononuclear cells from whole blood and DC generation Peripheral blood was obtained from healthy donors or from patients with uveal melanoma into heparin, and peripheral blood mononuclear cells (PBMC) isolated by standard gradient centrifugation in Lymphoprep (Nycomed, Pharmaceuticals Buckinghamshire, UK).

Techniques: Expressing, Activation Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay